human cervical carcinoma hela tet on cells (TaKaRa)
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TaKaRa
human cervical carcinoma hela tet on cells
Human Cervical Carcinoma Hela Tet On Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cervical carcinoma hela tet on cells/product/TaKaRa
Average 94 stars, based on 330 article reviews
Human Cervical Carcinoma Hela Tet On Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cervical carcinoma hela tet on cells/product/TaKaRa
Average 94 stars, based on 330 article reviews
human cervical carcinoma hela tet on cells - by Bioz Stars,
2026-02
94/100 stars
Images
1) Product Images from "Expression of Ebolavirus glycoprotein on the target cells enhances viral entry"
Article Title: Expression of Ebolavirus glycoprotein on the target cells enhances viral entry
Journal: Virology Journal
doi: 10.1186/1743-422X-6-75
Figure Legend Snippet: EGP expression in target cells enhances EGP/HIV transduction . (A) Cell surface expression of EGP. EGP Tet-On cells were seeded in 12-well plates (6 × 10 4 cells/well) and EGP expression was induced with indicated concentrations of dox. After 24 h post-induction, cell surface EGP levels were analyzed by flow cytometry using an EGP monoclonal antibody. (B) Western blot analysis of EGP expression in Tet-On cells. EGP Tet-On cells were seeded in 12-well plates and induced with indicated concentrations of dox. Forty-eight hours post-induction, cell lysates were subjected to SDS-PAGE followed by immunoblotting using a EGP monoclonal antibody. (C) HIV pseudotyping constructs. HIV packaging construct encodes gag/pol genes required for virion assembly. Envelope expression construct encodes genes for EGP or MGP or VSV-G under the control of a CMV promoter. HIV reporter construct encodes the viral genomic RNA, carrying a luciferase or a GFP reporter gene. (D) Enhancement of EGP/HIV transduction by EGP expression in target cells. EGP or control Tet-On cells were seeded in 24-well plates (3 × 10 4 cells/well) and induced with varying concentrations of dox. After 24 h post-induction, cells were challenged with EGP/HIV, MGP/HIV or VSV-G/HIV pseudovirions carrying a luciferase reporter gene. The luciferase activities in the cell lysates were measured 48 h post-infection and are presented as percentage of the uninduced cells (100%). Data represents an average of at least three independent experiments. Bars, standard deviations. (E) The mucin-like region in EGP is not required for enhancement. Tet-On stable cells with EGP mutant lacking the mucin-like region (ΔEGP) were induced with dox and challenged with pseudotyped virions carrying luciferase reporter. The luciferase activities are shown as relative percentage of the uninduced cells (100%). Data represents an average of at least three independent experiments. Bars, standard deviations. (F) EGP Tet-On cells infected with EGP/HIV pseudovirions carrying a GFP reporter. The percentage of GFP expressing cells, shown in each panel as inserts, were quantified by flow cytometry.
Techniques Used: Expressing, Transduction, Flow Cytometry, Western Blot, SDS Page, Construct, Luciferase, Infection, Mutagenesis
Figure Legend Snippet: GP enhancement is correlated with the entry susceptibility of the target cells . (A) EGP expression in HeLa cells enhances EGP/HIV transduction. HeLa Tet-On cells with EGP, ΔEGP or control vector were induced with dox and challenged with pseudotyped viruses. The luciferase activities were measured 48 h post-infection and are shown as percentage of the uninduced cells (100%). Data represents an average of at least three independent experiments. Bars, standard deviations. (B) EGP expression in Jurkat Tet-On cells does not enhance EGP/HIV transduction. Jurkat Tet-On cells expressing EGP or ΔEGP or control vector were challenged with luciferase reporter virus and luciferase activity in the cell lysates are shown in relative light units (RLU). Data represents an average of at least three independent experiments. Bars, standard deviations. (C) Enhancement of EGP/MLV pseudovirus transduction in HeLa Tet-On cells. HEK Tet-On cells with EGP, ΔEGP or control vector induced with dox were challenged with ΔEGP/MLV pseudovirion carrying a GFP reporter. The percentage of GFP expressing cells are shown as inserts in each panel.
Techniques Used: Expressing, Transduction, Plasmid Preparation, Luciferase, Infection, Activity Assay
Figure Legend Snippet: Analysis of Ebola GP mutants in trans enhancement process . (A) HEK Tet-On cells expressing Wt EGP were challenged with EGP mutant viruses, GP1 mutants, relative infectivity of Wt and GP1 mutant viruses. Fusion peptide mutants, relative infectivity of Wt and fusion peptide mutant viruses. Coiled-coil mutants, relative infectivity of Wt and GP2 mutant viruses. (B) Matrix analysis of trans enhancement by EGP. Wt or mutant EGP expressing HEK Tet-On was either uninduced or induced with 1 μg/ml of Dox. After 24 h, the cells challenged with pseudovirus particles carrying Wt or mutant EGP. The luciferase activities in infected cells are represented as relative percentage of luciferase activity in uninduced cells. Data represents an average of three independent experiments. For clarity of the chart we have omitted the error bars in panel B.
Techniques Used: Expressing, Mutagenesis, Infection, Luciferase, Activity Assay
